I bet you are wondering "CAN I GET ALL OF THIS DONE IN TIME TO BREED?......YES, you will have enough time to get it all in if you and your vet are prepared. The ovulation for each female is individual and can vary several days, which is why the vag swabs along with the progesterone testing are so important. Progestrone testing is the one way breeders can know when a female dog is ovulating, thus determining the ideal time to breed.
PROGESTERONE TESTING (highly recommended for fresh, chilled & frozen):
Progesterone is a reproductive hormone that begins to increase in the bloodstream just prior to ovulation. For most bitches a progestrone reading of 2.0 nanograms indicates a surge in the Luteinizing Hormone (LH) from the pituitary gland, which triggers the release of the eggs from the follicles. When Progesterone levels reach 5.0 to 10.0 a bitch is then ovulating.
* Progesterone levels from testing methods may very which is why a "quanative"
Progesterone level is recommended.The key is to get a baseline reading and see
an increase from the level to know the female is ovulating.
* Within the first 5 days of Proestrus, take a progesterone reading to determine the
baseline level (proestrus is commonly refered to as the last days or end of the
bleeding heat portion)
* At 50% cornification, or around the 4th or 5th day of Proestrus, start doing the
progesterone testing every other day.
* Around the time of LH surge is occuring, the progesterone level will be increasing
to (each female may vary this is why the vag swabs are helpful) above 2.5ng/ml
which usually means the ovulation has occured, and breeding my commence
within 48-72 hrs (depending on the type of breeding).
* When the progesterone level has risen to the level of 10ng/ml the female is during
her peak of fertility. PEAK IS CONSIDERED TO BE BETWEEN 5-20ng/ml on average.
* Progesterone will continue to remain elevated for 2-3 months in both pregnant and
Many Progesterone Kits Chart progesterone levels thru color changes and these types are used by many vets. IF YOU ARE PAYING FOR A STUD SERVICE......IT IS BETTER TO KNOW THE QUANTITATIVE PROGESTERONE LEVEL (an exact number) rather than being told to start breeding in a certian number of days and breed 2-3 times to allow for error. Color kits will give levels in C1-C5 each group repersenting a range of numbers. If the Quantitive Level (exact measurement in numbers) is not available at your vets check on the cost and expected turn around time to draw and
send it to another lab or location for the numeric count. Otherwise check with other
vets in your area to see if they offer a numeric reading, most fertility specialists have
this in-house as well as the larger vet clinics.
In cases where it takes longer than a couple hours to obtain a quanitative
progesterone level (such as needing to send to another lab after collection requiring a 24hr period or longer to obtain results), "breeders must use combination of the
laboratory test and behavorial changes to help pinpoint the time". To accuratley
identify the time of ovulation, a series of progesterone tests are conducted to map
the rise of progesterone levels. Multiple testing becomes even more critical when
chilled semen is being shipped to another state or country and when using frozen
semen. BOTH REQUIRE THE MOST PERCISE TIMING TO INCREASE THE POTENTIAL
OvuCHECK is the only approved color charted Progesterone Testing Kit that is approved by the Synbiotics Corporation Canine Reproduction Specialists and is developed and manufactured by Synbiotics Corporation and is available for purchase by your vet. *NEW VET ACCOUNTS can be established w/ Order. Sameday and next day shipping available via Fed-Ex
OVULATION OCCURS AFTER THE LH HORMONE SURGE
After ovulation, the released eggs continue to go through maturing process which ends 2-3 days later. ONCE THE MATURATION PROCESS IS COMPLETED......EGGS ARE READY FOR FERTILIZATION! As a result, the prime time to breed is 48 to 72 hours after ovulation!
Once Ovulation Occurs it takes between 48-72 hours for the eggs to become mature enough to fertilize and a male's sperm can survive safely up to 48 hours inside the female which is why it is important to be percise on your insemination date.
* Within the first 5 days of Proestrus, take a progesterone reading to determine a
* At 50% cornification, around the 4th or 5th day of the Proestrus (ed of bleeding heat),
start doing the LH testing daily (if available) and Progesterone testing every other day.
* A Positive Status-LH test result indicates the LH level in the sample is > 1ng/ml.
During this peak, progesterone levels are about 1.5 to 2.5 ng/ml. Around this time the
progesterone will be increasing.
* The day of the LH surge is counted as Day ). The surge will last approximatley 24
hours and triggers the ovulation. OVULATION OCCURS 2 days AFTER THE LH SURGE
The Fertile Period is 4-7 days post LH SURGE and lasts 48-72 hours. * Do another Progesterone test 2-4 days post LH surge to e sure that the progesterone is indeed increasing thus confirming that the ovulation has occured. Witness LH was designed to pinpoint the optimum time for canine breeding. Since inception, LH testing has proven variety of uses in
During the canine estrus cycle, the LH surge may occur from days 3 to 28 (average days 3-12) after obserable signs of heat and lasts approximately 24 hours. Ovulation occurs 2 days after the LH surge. The ova/eggs then require and additional 2-3 days to mature into a stage that will support fertilization.
The fertile period of the bitch falls between days 4 and 7 after the LH surge with the most fertile days being 5 and 6 (the day of the LH sure is counted as Day 0). Determining the LH surge is
perhaps the most critical factor in reproductive efficiency.